CLSI HA5 Collection, Transport, and Processing of Blood Specimens for Testing Plasma-Based Coagulation Assays and Molecular Hemostasis Assays;. CLSI HA5. Collection, Transport, and Processing of Blood Specimens for Testing Plasma-Based Coagulation Assays; Approved Guideline. HA5 does not provide general guidelines for the performance of coagulation testing. Performance guidelines for specific coagulation assays are addressed.

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A clear statement describing the magnitude and direction of the interference must be included. Similarly, brown coloured serum due to methaemalbumin, normal results for potassium or the same result from arterial and venous collections should prompt further investigation e. Laboratories must determine and document at which H-Index level recollection of the sample is appropriate.

Best Practices in Phlebotomy. The interference with immunoassays is less frequent than with photometric assays, but is still a significant consideration; one of the most commonly affected immunoassays are the cardiac Troponin assays.

You may delete a document from your Alert Profile at any time. No literary matter in The Clinical Biochemist — Reviews is to n reproduced, stored in a retrieval system or transmitted in any form by electronic or mechanical means, photocopying or recording, without permission. Laboratories must be familiar with best practice collection processes.

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Please review our privacy policy. We have no amendments or corrections for this standard. Support Center Support Center. This standard is not included in any packages. This decision should be guided by a policy developed in consultation with requesting clinicians.

Consensus Statement for the Management and Reporting of Haemolysed Specimens

The interference can be the result of various mechanisms including: Enzymatic – release of enzymes which degrade analytes e.

Clinically relevant limits can be sourced from: Requests to do so 2-1a5 be addressed to the Editor. As the voice of the U. The interference can be the result of various mechanisms including:.

Non standardisation of units. Introduction Haemolysis of blood samples has been documented as the most frequent pre-analytical artefact encountered by laboratories.

Your Alert Profile lists the documents that will be monitored. Where semi-quantitative estimates are in use, their equivalence to a conventional unit e. Ann Acad Med Singapore. Dilutional gross haemolysis releasing cell fluid and content – leading to false lowering of results e. Published clinical outcome studies describing performance requirements for the specific analyte.

The guideline provides procedures for the collection, transport, and processing of blood specimens for coagulation testing. When a POCT result shows a result e.

Example of a Haemolysis Colour Chart which also shows haemolysis index concentration levels across different analytical platforms Courtesy Goce Dimeski, Pathology Queensland. Author information Copyright and License information Disclaimer. The contents of articles or advertisements in The Clinical Biochemist — Reviews are not to be construed as official statements, evaluations or endorsements by the AACB, its official bodies or its agents.

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Clin Chem Lab Med.

Observational study to determine factors associated with blood sample haemolysis in the emergency department. Please first verify your email before subscribing to alerts. Haemolysis of blood samples has been documented as the most frequent pre-analytical artefact encountered by laboratories.

Clinically relevant limits can be sourced from:. Spectral 21-aa5 absorbs atand nm – leading to interference with alkaline phosphatase, gamma-glutamyl transferase, total and direct bilirubin. However, these guidelines may also be considered for other sample types e.

Consensus Statement for the Management and Reporting of Haemolysed Specimens

If a laboratory decides to report affected tests, the H-Index levels used for these purposes must be documented. Where multiple different analytical cli are in use, for example across a network of laboratories or in a large central laboratory, laboratories must ensure H-Index thresholds are appropriate for each.

Whole Blood Analyses S5. Proceed to Checkout Continue Shopping. Journal List Clin Biochem Rev v.